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Product Usage Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

CD69 (E1U2O) Rabbit mAb recognizes endogenous levels of total CD69 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu120 of human CD69 protein.

Background

CD69, also known as Leu-23, is a type II transmembrane glycoprotein that is expressed on the surface of T cells, B cells, and NK cells (1,2). This phosphorylated disulfide-linked 28 to 32-kDa homodimer is constitutively expressed on a subset of thymocytes and platelets. It also acts as an activation antigen of lymphocytes, NK cells, neutrophils, and eosinophils (1-6). Studies have shown that stimulation of the T cell receptor (TCR) increases the expression of CD69 on the cell surface. The ability to detect the level of CD69 expression after TCR activation makes CD69 an ideal indicator of T cell activation (1). 

1. Testi, R. et al. (1989) J Immunol 142, 1854-60.
2. Marzio, R. et al. (1997) J Leukoc Biol 62, 349-55.
3. Lanier, L.L. et al. (1988) J Exp Med 167, 1572-85.
4. Testi, R. et al. (1988) J Immunol 141, 2557-63.
5. Hartnell, A. et al. (1993) Immunology 80, 281-6.
6. Gavioli, R. et al. (1992) Cell Immunol 142, 186-96.