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Alternative Name:TLR2; CD282; TIL4; Toll/interleukin-1 receptor-like protein 4｜Application:Flow cytometry (Qualified)｜Brand:BD OptiBuild?｜Concentration:0.2 mg/ml｜Entrez Gene ID:7097｜Immunogen:Human TLR2-transfected cell line｜Isotype:Mouse IgG1, κ｜Reactivity:Human (Tested in Development)｜Regulatory Status:RUO｜RRID:AB_2873147｜Storage Buffer:Aqueous buffered solution containing ≤0.09% sodium azide.｜描述： The 11G7 monoclonal antibody specifically binds to human CD282, which is also known as Toll-like receptor 2 (TLR2). CD282 is expressed on monocytes, granulocytes, and dendritic cells. Toll-like receptors (TLRs) play a critical role in antimicrobial resistance. Moreover, TLRs have been shown to activate a number of signal transduction pathways which lead to the induction of genes involved in host defense. TLRs are type-1 transmembrane receptors characterized by the presence of extracellular leucine-rich repeat and intracellular Toll/IL-1 receptor domains. At least 12 mammalian TLRs have been identified, each recognizing a distinct bacterial or viral pathogen-associated molecular pattern, termed PAMP.??Peptidoglycan from Gram-positive bacteria, lipoproteins and lipopeptides from several bacteria, glycophosphatidylinositol, lipoarabinomannan, porins, and zymosan from yeast have been reported to be the ligands for TLR2.?? It has been reported that mAb 11G7 inhibits the production of inflammatory cytokines via certain TLR2 ligands including TLR2/TLR1 ligands, lipoarabinomannan and PAM3CSK4.??However, 11G7 antibody does not inhibit the production of inflammatory cytokines with zymosan, a TLR2/TLR6 ligand.??Please note that this application has not been tested at BD Biosciences Pharmingen. ｜商品通知：This antibody was developed for use in flow cytometry.The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.Researchers should determine the optimal concentration of this reagent for their individual applications.An isotype control should be used at the same concentration as the antibody of interest.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.｜推薦的實驗流程： For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.??Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.??The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.??More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).