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Alternative Name:Rat IgG2a kappa Isotype Control｜Application:Flow cytometry, Isotype control (Routinely Tested)｜Brand:BD Horizon?｜Immunogen:Mouse Pooled Immunoglobulin｜Isotype:Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ｜Regulatory Status:RUO｜RRID:AB_2869755｜Storage Buffer:Aqueous buffered solution containing ≤0.09% sodium azide.｜描述： The R35-95 hybridoma was generated by hybridization of Y3 myeloma cells with spleen cells from LOU rats immunized with mouse immunoglobulins. The R35-95 hybridoma produces rat IgG2a, κ immunoglobulin that has no measurable reactivity with mouse immunoglobulins. The R35-95 immunoglobulin was selected as an isotype control following screening for low background binding on a variety of mouse and human tissues. 商品通知：Since applications vary, each investigator should titrate the reagent to obtain optimal results.An isotype control should be used at the same concentration as the antibody of interest.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.For fluorochrome spectra and suitable instrument settings, BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.BD Horizon Brilliant? Violet 750 is covered by one or more of the following US patents: 8,158,444; 8,802,450; 8,575,303; 8,455,613; 8,227,187; 8,841,072; 8,110,673.｜推薦的實(shí)驗(yàn)流程：For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the Brilliant Stain Buffer (Cat. No. 563794/566349).