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Alternative Name:Ly-3; Lyt-3; Lymphocyte antigen 3; Ly-C; CD8b1｜Application:Flow cytometry (Qualified)｜Brand:BD OptiBuild?｜Concentration:0.2 mg/ml｜Entrez Gene ID:12526｜Immunogen:5-day MLR, C57BL/6 anti-BALB/c｜Isotype:Rat IgG2b, κ｜Reactivity:Mouse (Tested in Development)｜Regulatory Status:RUO｜RRID:AB_2872172｜Storage Buffer:Aqueous buffered solution containing ≤0.09% sodium azide.｜描述： The H35-17.2 monoclonal antibody specifically binds to both alloantigeneic forms of the β chain of the CD8 differentiation antigen (Ly-3 or Lyt- 3).??The CD8 α and α' chains (CD8a) form heterodimers with the CD8 β chain (CD8b, Ly-3, or Lyt-3) on the surface of most thymocytes.??A subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells) expresses almost exclusively the CD8 αβ heterodimer (the α' chain is absent).??Subsets of γδ TCR-bearing T cells, intestinal intraepithelial lymphocytes, and dendritic cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to T lymphocytes which matured in the thymus or in an extrathymic environment that had been influenced by thymus- initiated neuroendocrine signals.??CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck (p56lck). The H35-17.2 mAb blocks T-cell-mediated cytolysis of allogeneic lymphoma cells. ｜商品通知：This antibody was developed for use in flow cytometry.The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.Researchers should determine the optimal concentration of this reagent for their individual applications.An isotype control should be used at the same concentration as the antibody of interest.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.BD Horizon Brilliant? Violet 750 is covered by one or more of the following US patents: 8,158,444; 8,802,450; 8,575,303; 8,455,613; 8,227,187; 8,841,072; 8,110,673.｜推薦的實驗流程： For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.??Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.??The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.??More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).