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Alternative Name:Macrophage mannose receptor; MMR; MRC1; CLEC13D｜Application:Flow cytometry (Routinely Tested)｜Brand:BD Pharmingen?｜Immunogen:Human Placental Macrophage Mannose Receptor｜Isotype:Mouse IgG1, κ｜Reactivity:Human (QC Testing)｜Regulatory Status:RUO｜RRID:AB_398476｜Storage Buffer:Aqueous buffered solution containing BSA and ≤0.09% sodium azide.｜Vol. Per Test:20 μl｜Workshop Number:VII 70318｜描述： The 19.2 monoclonal antibody specifically binds to CD206 which is also known as the macrophage mannose receptor (MMR) or C-type lectin domain family 13 member D (CLEC13D). CD206 is a type I transmembrane glycoprotein of approximately 162 kDa which binds to glycoconjugates containing mannose, fucose, or N-acetylglucosamine. These carbohydrates are present on the surface of many microorganisms and enable the macrophage to bind to microorganisms through the MMR and internalize them during the process of phagocytosis. CD206 is expressed on human macrophages, endothelial cells, and cultured dendritic cells. It is not detected on resting monocytes. Mannose receptor expression is upregulated on peripheral blood mononuclear cells following 3 day incubation with GM-CSF. ｜商品通知：This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental sample (a test).An isotype control should be used at the same concentration as the antibody of interest.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.Source of all serum proteins is from USDA inspected abattoirs located in the United States.For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at .This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.｜推薦的實(shí)驗(yàn)流程：BD? CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD? CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD? CompBeads to ensure that BD? CompBeads are appropriate for your specific cellular application.