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Alternative Name:IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55｜Application:Flow cytometry (Routinely Tested)｜Brand:BD Pharmingen?｜Entrez Gene ID:3559｜Immunogen:Phytohemagglutinin stimulated human lymphocytes｜Isotype:Mouse BALB/c IgG1, κ｜Reactivity:Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)｜Regulatory Status:RUO｜Storage Buffer:Aqueous buffered solution containing BSA and ≤0.09% sodium azide.｜Vol. Per Test:5 μl｜Workshop Number:IV A053｜描述： The M-A251 monoclonal antibody specifically binds to the 55 kDa type I transmembrane glycoprotein known as??low-affinity interleukin-2 receptor alpha chain subunit (IL-2Rα). CD25 is expressed on regulatory T cells,??activated lymphocytes (T and B), and monocytes. It associates with the IL-2Rβ/CD122 and IL-2Rγ/CD132 receptor chains to form the high-affinity IL-2R complex. CD25 expression on T and B lymphocytes is upregulated by antigenic or mitogenic stimulation. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. ｜商品通知：This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental sample (a test).An isotype control should be used at the same concentration as the antibody of interest.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.Source of all serum proteins is from USDA inspected abattoirs located in the United States.Alexa Fluor? 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.Alexa Fluor? is a trademark of Life Technologies Corporation.Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.｜推薦的實(shí)驗(yàn)流程： BD? CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD? CompBeads, they have spectral properties very similar to cells.??However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD? CompBeads to ensure that BD? CompBeads are appropriate for your specific cellular application.