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Alternative Name:B7.2; B7-2; B-lymphocyte activation antigen B7-2; B70; BU63; CD28LG2; LAB72｜Application:Flow cytometry (Routinely Tested)｜Brand:BD Horizon?｜Immunogen:Human HBL-1 Cell Line｜Isotype:Mouse BALB/c IgG1, κ｜Reactivity:Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)｜Regulatory Status:RUO｜RRID:AB_2739530｜Storage Buffer:Aqueous buffered solution containing BSA and ≤0.09% sodium azide.｜Vol. Per Test:5 μl｜Workshop Number:V B046, BP126｜描述： The 2331 (FUN-1) monoclonal antibody specifically recognizes a 75 kDa transmembrane cell surface protein, CD86 (B70/B7-2), expressed primarily on monocytes, dendritic cells and activated B cells. Competitive binding assays demonstrate that, while both 2331 (FUN-1) and IT2.2 (Anti-CD86) antibodies specifically recognize the same molecule, they react with different epitopes. CD86 is a ligand for CD28 and CTLA-4 and plays an important role in costimulation of T cells in primary immune response. The 2331 (FUN-1) antibody blocks the costimulatory activity of CD86 when tested in functional studies. ｜商品通知：This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental sample (a test).An isotype control should be used at the same concentration as the antibody of interest.Source of all serum proteins is from USDA inspected abattoirs located in the United States.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.BD Horizon Brilliant Violet 480 is covered by one or more of the following US patents: 8,575,303; 8,354,239.For fluorochrome spectra and suitable instrument settings, ｜推薦的實(shí)驗(yàn)流程：For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).