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Alternative Name:Cd8b1; Cd8b; Ly-3; Lyt-3; lymphocyte antigen 3｜Application:Flow cytometry (Qualified)｜Brand:BD OptiBuild?｜Concentration:0.2 mg/ml｜Entrez Gene ID:12526｜Immunogen:Mouse Thymocytes｜Isotype:Rat IgG1, κ｜Reactivity:Mouse (Tested in Development)｜Regulatory Status:RUO｜Storage Buffer:Aqueous buffered solution containing ≤0.09% sodium azide.｜描述： The YTS156.7.7.rMAb is a recombinant monoclonal antibody derived from YTS156.7.7 hybridoma cells that specifically recognizes the β chain of the CD8 differentiation antigen (CD8b), which is also known as Lymphocyte antigen 3 (Ly-3) or Lyt-3. CD8b is a single-pass type I transmembrane glycoprotein that is encoded by Cd8b1 (CD8 antigen, beta chain 1) which belongs to the Ig superfamily. CD8b is comprised of an N-terminal IgV domain in its extracellular region followed by a transmembrane region and a cytoplasmic tail. CD8b can bind to the CD8 α chain (CD8a) to form the disulfide-linked CD8 αβ (CD8ab) heterodimer also known as Ly2,3 or Lyt-2,3. This heterodimer is expressed on most thymocytes and a subpopulation of MHC class I-restricted mature T cells. CD8 αβ functions as an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and the protein tyrosine kinase, lck (p56lck). ｜商品通知：Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.Since applications vary, each investigator should titrate the reagent to obtain optimal results.The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant? Stain Buffer in your experiments to help mitigate potential background. When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse? Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS? Lysing Solution (Cat. No. 349202) after staining.For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page An isotype control should be used at the same concentration as the antibody of interest.Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.｜推薦的實驗流程： BD? CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD? CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD? CompBeads to ensure that BD? CompBeads are appropriate for your specific cellular application.