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Alternative Name:Neuropilin; NRP; NP1; Blood dendritic cell antigen 4; BDCA-4; VEGF165R｜Application:Flow cytometry (Qualified)｜Brand:BD OptiBuild?｜Concentration:0.2 mg/ml｜Immunogen:Recombinant Human Neuropilin-1｜Isotype:Mouse IgG2b, κ｜Reactivity:Human (Tested in Development)｜Regulatory Status:RUO｜Storage Buffer:Aqueous buffered solution containing ≤0.09% sodium azide.｜描述： The U21-1283 monoclonal antibody specifically recognizes Neuropilin-1 (NRP1), also known as CD304, Blood dendritic cell antigen 4 (BDCA4), and Vascular endothelial cell growth factor 165 receptor (VEGF165R). CD304 is a type I transmembrane glycoprotein involved in the development of the nervous and cardiovascular systems. It mediates the interaction, growth, survival, and migration of a variety of normal and tumor cells. CD304 is expressed on neurons, thymocytes, regulatory T cells, a subset of T follicular helper cells, dendritic cells, endothelial cells, and certain tumor cells. Neuropilin-1 has a very short cytoplasmic domain and interacts with various coreceptors to form ligand-binding, signal-transducing receptor complexes. CD304 complexes with Plexin-A family members to bind chemorepellent Class 3 Semaphorins and guide neuronal axon growth. It also functions as a coreceptor with VEGFR2/CD309 to stimulate angiogenesis in response to VEGF165. CD304 mediates the interactions between some T cells and dendritic cells. ｜商品通知：Researchers should determine the optimal concentration of this reagent for their individual applications.The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.An isotype control should be used at the same concentration as the antibody of interest.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.CF? is a trademark of Biotium, Inc.For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page Since applications vary, each investigator should titrate the reagent to obtain optimal results.Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant? Stain Buffer in your experiments to help mitigate potential background. ｜推薦的實(shí)驗(yàn)流程： BD? CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD? CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD? CompBeads to ensure that BD? CompBeads are appropriate for your specific cellular application.