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Product Usage Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

CD200 (E5I9V) XP^? Rabbit mAb recognizes endogenous levels of total CD200 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with human CD200 recombinant protein and reacts with an epitope surrounding Ala164.

Background

CD200 (OX2) and CD200R (OX2R) are membrane glycoprotein members of the Ig superfamily (1-3). CD200 is expressed by a range of cells, including neurons, epithelial cells, endothelial cells, fibroblasts, and lymphoid cells, while its receptor, CD200R, is found on myeloid and T cells (1-5). Interaction between CD200 and CD200R downregulates macrophage function and plays a role in immunosuppression and regulation of anti-tumor immune responses (3-7).

1. Barclay, A.N. (1981) Immunology 44, 727-36.
2. McCaughan, G.W. et al. (1987) Immunogenetics 25, 329-35.
3. Wright, G.J. et al. (2000) Immunity 13, 233-42.
4. Hoek, R.M. et al. (2000) Science 290, 1768-71.
5. Wright, G.J. et al. (2003) J Immunol 171, 3034-46.
6. Kretz-Rommel, A. et al. (2007) J Immunol 178, 5595-605.
7. Fallarino, F. et al. (2004) J Immunol 173, 3748-54.